1992 May;73 ( Pt 5):1159-65. doi: 10.1099/0022-1317-73-5-1159. However, some differences from pH 7.2 can be observed from the tomograms. Recent study reveals broad-spectrum antiviral properties of Andro . For more information about this format, please see the Archive Torrents collection. In developing pandemic vaccine viruses, reverse genetics has been employed as a rational approach that can also be used effectively to attenuate the highly virulent H5N1 virus and at the same time place the H5 HA and N1 NA on a background of PR8, a virus . For additional information, or to request that your IP address be unblocked, please send an email to PMC. Support Center Find answers to questions about products, access, use, setup, and administration. Sedimentation studies in sucrose solutions of varying densities showed a non-linear dependence of sedimentation rate upon solvent density, indicating that the density in solution increases with increasing sugar concentration. ( A) An influenza viral particle diffuses to the polycation-coated surface from solution and ( B) adheres to it. sharing sensitive information, make sure youre on a federal Abstract Highly purified preparations of PR8 influenza virus were obtained from perfused, infected mouse lungs by a combination of methods involving adsorption of the virus on and elution from chicken red cells and differential centrifugation. Bethesda, MD 20894, Web Policies 5. C3m was not effective against H1N1 pr8 influenza A virus, which binds to glycosylated oligosaccharides that terminate in a sialic acid , suggesting that the C3m effect is specific to ICAM-1-mediated effects on viral replication. . The virus particles floated in a sugar solution with a density of 1.18. The spreading of the boundary during sedimentation was shown to be represented by a standard deviation of the sedimentation rate equal to 8 per cent of the mean. concentrated and purified from infectious allantoic fluids by means of one or two cycles of differential centrifugation and inactivated by different treatments have been prepared and subjected to laboratory tests. Phylogenetic analysis revealed that the genome sequences of . To update the vaccine strains in the antigen bank, we used reverse genetics technology to develop two vaccine candidates against avian influenza strains belonging to clades 2.3.2.1d and 2.3.4.4h, and then evaluated their immunogenicity and . Register or Login to get started. A more slowly sedimenting component was observed with the ultracentrifuge in the highest concentrations of several of the preparations. Bethesda, MD 20894, Web Policies In certain embodiments, the molecule comprises at least one 5-triphosphate and/or at least one 5-diphosphate. official website and that any information you provide is encrypted 1943 Dec 31;98(2557):587-9 The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. government site. -, J Exp Med. Rovnova ZI, Isaeva EI, Platonova AL, Rogacheva TA. Welcome to the Bacterial and Viral Bioinformatics Resource Center (BV-BRC), an information system designed to support research on bacterial and viral infectious diseases. official website and that any information you provide is encrypted Conformational changes in myosin and heavy meromyosin from chicken gizzard associated with phosphorylation. Preparation of inactivated influenza vaccines is conventionally achieved by exposure of cell- or egg-derived live virus to beta-propiolactone (BPL) or formaldehyde (FA). WSN, PR8 and P09 viruses were inhibited by SERINC5, whereas the H3N2 virus was resistant (Fig 4G). and transmitted securely. Influenza A virus replication cycle The RNA segments of the viral genome have complementary base sequences at the terminal ends, allowing them to bond to each other with hydrogen bonds. After purification by density gradient procedures, the virus preparations were tested for the presence of host antigens with antisera to appropriate host materials. Product category Viruses Product type J Exp Med. These types of effects would disrupt the ordered lipid rafts ( 32, 33) and leaflet asymmetry ( 34) in influenza viral envelopes. In this study, we detected three H12 IAVs from Anascrecca and Anas formosa in Poyang Lake, China, in 2018, one of which was isolated. The specific volume was determined with a pycnometer to be 0.79. This project has been funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. J Exp Med. [Identification of the antigenic components of the host cell in isolated glycoproteins of the influenza virus]. The extrapolated value for one preparation was 722 Svedberg units and the value for another preparation was 658 units. ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS. The CPSF inhibitory properties of NS1 appear to be dispensable for some influenza virus strains . Apigenin suppresses influenza A virus-induced RIG-I activation and viral replication Flavonoid apigenin is low toxicity and few favorable bioactivities, including anti-tumor properties, antioxidant, anti-inflammatory activity and antiviral. PMC The intrinsic viscosities, calculated on the basis of concentrations expressed as grams per cubic centimeter, of several highly purified preparations were determined to be between 11.3 and 16.5. Introduction Although aluminum in virtually all currently adjuvant human vaccines is used to help increase the humoral or cellular immune responses to an antigen, it usually induces a Th2 antibody dominated response. Elford, Andrewes, and Tang (1) obtained a filtration end-point of 160 m # for the W.S. Max A. Lauffer, W. M. Stanley; BIOPHYSICAL PROPERTIES OF PREPARATIONS OF PR8 INFLUENZA VIRUS . Evidence is reported which suggests that cellular actin is an integral component of highly purified PR8 virions, though probably unrelated to host antigen. PLOS Pathogens publishes Open Access research and commentary that significantly advance the understanding of pathogens and how they interact with host organisms.. Get Started Epub 2015 Jan 19. The biophysical properties of several preparations of PR8 influenza virus have been studied. Peer-reviewed Research Article Antibodies with 'Original Antigenic Sin' Properties Are Valuable Components of Secondary Immune Responses to Influenza Viruses Susanne L. Linderman, AffiliationsDepartment of Microbiology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, The .gov means its official. Please contact us if you have any questions or issues. J Virol. By contrast with SP-D, human SP-A also has considerable HA inhibitory activity for PR8 (Hartshorn, manuscript submitted). Viral data and analysis tools and services from IRD/ViPR have now been integrated into the resource. MeSH Clipboard, Search History, and several other advanced features are temporarily unavailable. Taxonomy information for Influenza A virus (A/Puerto Rico/8/1934(H1N1)). A combination of high-yield mutations from these screens led to a PR8 backbone that improved the titres of H1N1, H3N2, H5N1 and H7N9 vaccine viruses in African green monkey kidney and. J Exp Med. FOIA La Biblioteca Virtual en Salud es una coleccin de fuentes de informacin cientfica y tcnica en salud organizada y almacenada en formato electrnico en la Regin de Amrica Latina y el Caribe, accesible de forma universal en Internet de modo compatible con las bases internacionales. Attenuated negative strand viruses with altered interferon antagonist activity for use as vaccines and pharmaceuticalsAttenuated negative strand viruses with altered interferon antagonist activity for use as vaccines and pharmaceuticals .. .. . Although both PR8 and CA04 belong to H1N1 IAV, they have distinct origins ( Califano et al., 2018 ). This virus was named Influenza B and the inactivated. Figure 2. Seasonal influenza infection is associated with 200,000-500,000 deaths each year, particularly in young children, immunocompromised patients and the elderly. PMC Would you like email updates of new search results? INFLUENCE OF pH AND OF CERTAIN OTHER CONDITIONS ON THE STABILITY OF THE INFECTIVITY AND RED CELL AGGLUTINATING ACTIVITY OF INFLUENZA VIRUS. -, J Exp Med. The density in the absence of sucrose was estimated to be about 1.1. The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. J Exp Med. MeSH The virus was shown not to lose infectivity very rapidly in sucrose solutions. Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 mmicro and a standard deviation of the distribution of diameters of 15 per cent. 1944 Mar 1;79(3):285-90 Morphologial and structural analysis of influenza virus PR8 strain at pH 5.5 In this condition, most virions have no apparent structural changes ( Fig. Accessibility Type I interferon regulates proteolysis by macrophages to prevent immunopathology following viral infection 2015 Mar 2;199:9-19. doi: 10.1016/j.virusres.2015.01.005. Bradshaw GL, Schlesinger RW, Schwartz CD. HHS Vulnerability Disclosure, Help The .gov means its official. If you have questions or need assistance, please contact us at help@bv-brc.org. 75N93019C00076, awarded to the University of Chicago. . The discrepancy between this value and that obtained from the electron micrographs, the greater size distribution from the electron micrographs, the slight irregularities in some of the particles as observed in the electron micrographs, the behavior of the sedimentation process in sucrose solutions of different densities, and the inactivation of the virus by withdrawal of electrolytes can all be explained in a straightforward manner if it is assumed that the virus particles in solution contain, in addition to the constituents shown by chemical analysis, about 60 per cent by weight of water. . The variation of sedimentation rate with concentration was shown to be due entirely to the variation of solution viscosity with concentration. Herpes simplex virus can similarly interact with N-linked oligosaccharides on SP-A . This drug-induced variant (DIV) has the following properties: 1. We determined the sensitivity of the influenza virus strain H1N1 (A/PR8/34) and the formerly pandemic H3N2 (A/Aichi/2/68) to carrageenans of subtypes iota and kappa by plaque reduction assays in MDCK cells .The results are summarized in Table 1.The two carrageenan types inhibited plaque formation of both viruses . Evaluation by immunoelectrophoretic methods. We used PR8 virus as our model virus to address this question. First, the density of the spike proteins has become blurred in some localized areas of the envelope. 8600 Rockville Pike Interleukin-7R in the regulation of lymphoid anti-viral responses and homeostasis My Research and Language Selection Sign into My Research Create My Research Account English; Help and support. Brief exposure of PR8 influenza virus at pH 4.5 to extracts of Aspergillus niger abolished the red cell . Upload and analyze your data in the private workspace. PRECIPITIN REACTIONS OF HIGHLY PURIFIED INFLUENZA VIRUSES AND RELATED MATERIALS. The specific volume was determined with a pycnometer to be 0.79. J Exp Med 1 December 1944; 80 (6): 531548. The virus particles floated in a sugar solution with a density of 1.18. Careers. The average particle diameter of the anhydrous PR8 virus particle was calculated from sedimentation and specific volume data to be about 80 m. 1944 Dec 1;80(6):507-20. doi: 10.1084/jem.80.6.507. As a result, all infected mice died within 9 days postinfection. For example, infection of mice with the pathogenic PR8 strain of influenza virus (A/Puerto Rico/8/1934 [H1N1]) at a sublethal dose results in spread of the virus to the heart and replication to low levels ( 9, 10 ), but the resulting myocarditis is mild and resolves quickly as the virus is cleared ( 9 ). The discrepancy between this value and that obtained from the electron micrographs, the greater size distribution from the electron micrographs, the slight irregularities in some of the particles as observed in the electron micrographs, the behavior of the sedimentation process in sucrose solutions of different densities, and the inactivation of the virus by withdrawal of electrolytes can all be explained in a straightforward manner if it is assumed that the virus particles in solution contain, in addition to the constituents shown by chemical analysis, about 60 per cent by weight of water. Modification du fond gntique PR8 pour l'optimisation qualitative et . Disclaimer, National Library of Medicine J Exp Med. Influenza viruses cause annual influenza epidemics and occasional pandemics, which pose a significant threat to public health worldwide. Bookshelf MeClelland, L., Canadian Public Health Journal 32: 530 (1941). Clipboard, Search History, and several other advanced features are temporarily unavailable. The extrapolated value for one preparation was 722 Svedberg units and the value for another preparation was 658 units. and transmitted securely. To identify Ab-dependent properties and serum components that contributed to enhancement of Ab activity, we screened a large panel of HA-specific MAbs for hemagglutination inhibition (HI) in the presence of noninactivated serum from naive mice (NMS). The migration of the data, tools, and services from the legacy IRD and ViPR applications to BV-BRC is now complete. Influenza A_N1_antiviral-response_278(1)_E258Q_Resistance to Oseltamivir: 278Q: No: Introduction of Glu258Gln naturally occurring substitution in the A/Vietnam/1203/2004 backbone decreased oseltamivir sensitivity using plaque reduction assay in MDCK cells. CENTRIFUGATION AND ULTRAFILTRATION STUDIES ON ALLANTOIC FLUID PREPARATIONS OF INFLUENZA VIRUS. The immunogenicity in mice of virus that had been exposed to A. niger extract appeared to be very broad, since vaccination of mice with such material gave protection against challenge with strains of influenza A virus representing all major serotypes as well as one type B influenza virus. In the severe influenza model group (500 PFU), 3 of. [26] FOIA Before The viscosity of the virus preparations can be explained as being due in great part to the presence of the slower sedimenting contaminating components which possess a very high intrinsic viscosity. The average particle diameter of the anhydrous PR8 virus particle was calculated from sedimentation and specific volume data to be about 80 mmicro. The inhibitory activity for several strains, including PR8, is not inhibited by EDTA or by sugars expected to block collectin activity. . The following materials are designed to aid IRD and ViPR users in using BV-BRC. 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